Trichototaxis songi细胞皮层色素颗粒的显微和亚显微观察

应用微分干涉相差显微术、扫描电镜和透射电镜显微术等显示了腹毛目纤毛虫Trichototaxis songi细胞形态及细胞皮层结构,观察到：细胞皮层存在红色色素颗粒和无色颗粒两种皮层颗粒．皮层色素颗粒与纤毛虫细胞呈现红色有关,它们使细胞的颜色保持不变．作者认为,这些色素颗粒可能已成为细胞皮层结构的基本成分,与纤毛器的功能作用有关;并且它们很可能是一类共生于纤毛虫细胞皮层内及皮层表面的细菌共生体．     

Secretory granules

Conclusions Although considerable knowledge has been acquired concerning the chemical nature of insulin and its pathway of biosynthesis, relatively little is known about the other proteins which constitute the insulin secretory granule. This, in part, has been a consequence of the difficulties of working with pancreatic islets as a tissue source and the attendant problems of obtaining sufficient material of suitable purity for experimental purposes. The scope for further investigation is perhaps best illustrated by the fact that of the 150 or so insulin granule proteins which may be visualized by electrophoresis the identity of less than 10 has been established. It is clear that some of the proteins will be shared by other granule types in accordance with the common cellular pathways involved in granule morphogenesis and exocytotic secretion. Equally though, it is also apparent that many of the peptides of the insulin granule are tailored for specific functions within the B-cell.     

冷冻干燥法和高速搅拌法制备生脉饮颗粒剂的研究

为了改善传统的中药制剂、方便服用，同时提高病人的顺应性，以传统的生脉饮制剂为模型药物，将其处方药材浸出液进行冷冻干燥，干燥物与不同的辅料混合，用高速搅拌粒法制成颗粒剂。结果表明，选择微粉硅胶与双歧糖为颗粒剂辅料，与浸膏干燥物的混合比例为1：3，再加入粘合剂10％HPMC的80％醇溶液4．0mL，即可使混合制粒顺利进行，粒度均匀，吸湿量低。因此，可以采用该方法制备中药颗粒剂，防止中药浸膏粉吸潮，提高中药颗粒剂的稳定性。     

信息系统中熵度量的粒化单调性

研究了不同二元关系下信息系统中信息粒的刻画和表示,引入了偏序关系,′发展了信息系统中的熵理论,证明了这些熵度量的粒化单调性.     

长毛对虾卵母细胞环形片层的研究

应用电镜技术研究长毛对虾(Penaeus penicillatus)卵母细胞中的环形片层(AL)。AL在卵黄发生前的卵母细胞卵质中出现,在卵黄发生期间消失。AL由8条平行排列,具有50～70 nm 环孔的片层组成。相邻片层之间的距离约为70 nm。AL 通常没有核糖体附在膜片层的外表面。AL末端膨大,与高尔基体毗邻。从形态学上看,AL可能来源于高尔基体;高尔基膜囊伸直、拉平,并局部融合形成环孔,最后演变成AL。卵黄发生期间,AL分化形成为许多囊泡,为卵黄粒的形成提供沉积的场所。本文还讨论高尔基体、AL 及卵黄粒形成之间的关系。     

Perforin and its role in T lymphocyte-mediated cytolysis

The killing mediated by cytotoxic T lymphocytes (CTL) represents an important mechanism in the immune defence against tumors and virus infections. The lytic mechanism has been proposed to consist of a polarized secretion of granule-stored molecules, occurring on effector-target cell contact. By electron microscopy, membrane deposited, pore-like lesions are detected on the target cell membrane during cytolysis by CTL. These structures resembled strikingly pores formed during complement attack.Granules of CTL isolated by nitrogen cavitation and Percoll gradient centrifugation were shown to retain cytotoxic activity. Further purification of proteins stored in these granules led to the discovery of a membranolytic protein named perforin which was capable of polymerizing into pore-like structures. In addition to this cytolytic protein, a set of serine esterases was found as well as lysosomal enzymes and proteoglycans, whose function are not yet clearly defined. The role of perforin in the cytotoxic process is currently being explored by ablating the active gene in mice.     

星点设计-效应面法优选淋必通颗粒的成型工艺

为了优选淋必通颗粒的成型工艺，以成型率、休止角、吸湿率、溶化性4项的总评OD值作为总评价指标，以辅料用量、乙醇体积分数、乙醇使用量作为考察因素，以星点设计-效应面法优选出淋必通颗粒的成型工艺。结果表明，淋必通颗粒的最佳成型工艺条件为m(干膏):m(糊精）为1:1，乙醇体积分数为90.0%，乙醇用量为每10.0 g干膏粉加90.0%的乙醇6.0 g。总评OD值均值为0.740 1，与预测值0.766 3相当。该方法预测性强，稳定性好，制备的淋必通颗粒具有成型率高、流动性好、抗吸湿性强及溶化性好的特点，为该复方制剂的工业化生产提供了理论参考。     

三偏磷酸钠交联木薯淀粉颗粒膨胀历程及结构特征

利用木薯淀粉与三偏磷酸钠的交联反应,通过控制交联反应程度,成功地控制了交联淀粉颗粒的膨胀程度并使其停留在不同的溶胀阶段,详细地研究了处在不同溶胀阶段的三偏磷酸钠交联木薯淀粉颗粒的结构特征和变化趋势,揭示了三偏磷酸钠交联木薯淀粉颗粒的具体的膨胀历程及溶胀机理,即交联木薯淀粉是从高交联时在中心脐点处的爆裂式膨胀,到低交联时颗粒首、尾端同时均衡发生溶胀的膨胀历程．     

灵芝对小鼠免疫功能调节作用的研究

报道了灵芝对ＢＡＬＢ／ｃ小鼠产生抗绵羊红细胞（ＳＲＢＣ）抗体的能力,对 性变态反应（ＤＨＴ）等免疫功能的影响。实验小鼠分为高、中、低剂量组、经胃灌注不同剂量的发芝浸液,每天五次连续灌１５ｄ,对照组自由摄水。用ＳＲＢＣ免疫小鼠,在免疫后第５ｄ用溶血空斑试验（ＰＦＣ）进行体生成细胞检测,以反映Ｂ细胞生成ＩｇＭ抗体的能力;于免疫后第４ｄ用足底厚法测定ＤＨＴ强度,以检测Ｔ细胞功能,用碳粒廓清检测巨噬细胞蚕     

蒸汽相中多孔玻璃粉自转晶含硼CF-2沸石的制备与表征

报道了在最小的有机胺--甲胺和水的蒸汽相中,多孔玻璃粉自转晶为含硼CF-2沸石. XRD、11B MAS NMR、SEM表征结果证明合成的CF-2沸石结晶度高且无杂晶,硼原子基本上都已进入了沸石骨架. 扫描电镜照片还显示,合成的CF-2沸石中既有单个晶体,也有由许多单个晶体构成的扫帚形聚集体. 单个晶体呈长条形,高、宽为0.30～1.1 μm,长为1.1～22 μm.